Journal: Folia Histochemica et Cytobiologica

Article Title: Distribution of cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) in the pterygopalatine ganglion of the domestic duck (Anas platyrhynchos f. domestica)

doi: 10.5603/fhc.a2016.0002

Figure Lengend Snippet: Figure 2. Immunoreactivity of neuropeptides in superior (SPPG) and inferior (IPPG) pterygopalatine ganglion of the domestic duck. A, B. High cocaine- and amphetamine-regulated transcript (CART)-immunoreactivity (IR) in ganglio- nic neurons (mainly in perikarya); C, D. Neuropeptide Y (NPY)-IR in numerous varicose fibers and in few ganglionic perikarya; E, F. Irregularly distributed galanin (GAL)-IR ganglionic neurons on the cross-sections through the ganglion. Sections were stained by simple immunofluorescence technique as described in Material and methods. Abbreviations: HG — Harderian gland

Article Snippet: The sections were incubated for 16 h at room temperature with primary mouse monoclonal antibodies directed against CART (1:2,000, MAB163, R&D Systems, Minneapolis, MN, USA) and GAL (1:2,000, MAB5854, R&D Systems), and rabbit polyclonal antibody directed against NPY (1:2,000, AHP 2189, AbD Serotec, Kidlington, UK).

Techniques: Staining, Immunofluorescence